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A novel molecular mechanism modulating osteoclast differentiation and function

✍ Scribed by H Yasuda; N Shima; N Nakagawa; K Yamaguchi; M Kinosaki; M Goto; S.-I Mochizuki; E Tsuda; T Morinaga; N Udagawa; N Takahashi; T Suda; K Higashio

Book ID
117808901
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
182 KB
Volume
25
Category
Article
ISSN
8756-3282

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## Abstract Osteoclasts are bone‐resorbing multinucleated cells differentiated from monocyte/macrophage lineage precursors. A novel osteoclast precursor cell line, 4B12 was established from Mac‐1^+^c‐Fms^+^RANK^+^ cells from calvaria of 14‐day‐old mouse embryos using __immunofluorescence and__ cell

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## Abstract Microarray and real‐time RT‐PCR were used to examine expression changes in primary bone marrow cells and RAW 264.7 cells in response to RANKL. In silico sequence analysis was performed on a novel gene which we designate OC‐STAMP. Specific siRNA and antibodies were used to inhibit OC‐STA