A Novel Method for the Preparation of Nucleoside Triphosphates from Activated Nucleoside Phosphoramidates
โ Scribed by Wu, Weidong; Freel Meyers, Caren L.; Borch, Richard F.
- Book ID
- 126989944
- Publisher
- American Chemical Society
- Year
- 2004
- Tongue
- English
- Weight
- 78 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1523-7060
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The importance of nucleoside 5'-di-and triphosphates for the synthetic processes of the cell has led to the development of numerous chemical (l-3) and enzymic (47) methods for their preparation. Although these methods have obvious advantages, each is subject to limitations. Thus, the chemical proce
A novel spectrophotometric method, based upon Raman spectroscopy, has been developed for accurate quantitative determination of nucleoside triphosphate phosphohydrolase (NTPase) activity. The method relies upon simultaneous measurement in real time of the intensities of Raman marker bands diagnostic