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A novel method for increasing the expression level of recombinant proteins

✍ Scribed by Aijun Wang; Jonathan Clapper; Jeffery A Guderian; Teresa M Foy; Gary R Fanger; Marc W Retter; Yasir A.W Skeiky

Book ID
117702746
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
515 KB
Volume
30
Category
Article
ISSN
1046-5928

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New vectors for high level expression of
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✍ David J. Hakes; Jack E. Dixon πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 860 KB

A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase (D. B. Smith and K. S. Johnson, 1988, Gene 67, 31-40). Expressed fusion proteins are purified from bacterial extracts by glutathione-agarose affinity chromat