✦ LIBER ✦

A novel method for identifying PEGylation sites of protein using biotinylated PEG derivatives

✍ Scribed by Haeshin Lee; Tae Gwan Park

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 124 KB
Volume: 92
Category: Article
ISSN: 0022-3549
DOI: 10.1002/jps.10270

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✦ Synopsis

The identification of PEGylation sites is essential in the characterization of PEGylated therapeutic proteins. This report describes a simple and novel method of finding poly(ethylene glycol) (PEG) conjugation sites in PEGylated proteins by using a hetero-functional biotin-PEG-N-hydroxyl succinimide derivative. PEGylated lysozyme species having a biotin moiety at each PEG chain end were separated and digested by trypsin. Among the digested lysozyme fragments, biotin-terminated PEGylated peptide fragments were purified by a monomeric avidin immobilized column. Their mass was analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry, directly indicating that PEG was conjugated to lysine 33, 97, 116 residues. Reversed-phase high-pressure liquid chromatography results for the PEGylated peptide fragments exhibited that PEGylation occurred preferentially at lysine 33> lysine 97> lysine 116.

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