A novel iterative optimizing quantization technique and its application to X-ray tomographic microscopy for three-dimensional reconstruction from a limited number of views
✍ Scribed by Heung-Rae Lee; Luiz DaSilva; Gary Ford; Waleed Haddad; Ian McNulty; James Trebes; Yin Yeh
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 254 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0899-9457
No coin nor oath required. For personal study only.
✦ Synopsis
The iterative optimizing quantization technique (IOQT) of the imaging source to use in a tomographic microscope has is a novel method in reconstructing three-dimensional (3D) images strongly depended on the nature of the interaction of the source from a limited number of 2D projections. IOQT can reduce the artifacts with the specimen material. Especially in recent years, substantial and image distortion due to a limited number of projections and limprogress has been made in XTM technology toward achieving ited range of viewing angles. Equivalently, by reducing the number nondestructive and high-resolution 3D X-ray microscope systems of projections required for reconstruction, the use of IOQT can reduce for biological application. A significant concern during this progthe dose delivered to the specimen, simplify the complexity of an ress is to determine the optimal X-ray wavelength range required experimental setup, and consequently support the development of in a given application to minimize the absorbed dose and still techniques to nondestructively image microstructures of materials. In achieve good contrast [1][2][3][4]. As a consequence, it has been found this article, we will demonstrate the capability of IOQT to reconstruct an accurate 3D image of an object from a limited number of views, that between the wavelengths of 2.3 and 4.4 nm, the absorption using a computer simulation and an actual 3D test pattern experiment cross section of oxygen is far smaller than that of carbon. This with submicrometer features. In addition, we will introduce a promisregion of the X-ray spectrum is defined as the water window, ing application of IOQT to X-ray tomographic microscopy to study which is the motivation for the use of soft X-rays to image living microbiological specimens by presenting the 3D reconstructions of specimens. In addition, studies for a maximal X-ray dosage to the two different-conditioned human sperm cells from six projections.