A novel in vitro model of human implantation: an endometrium-like three-dimensional (3D) culture system for attachment/invasion of trophoblast-like cells

Three step derivation of cartilage like

Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds

✍ Hai Yan Bai; Gui An Chen; Gen Hong Mao; Tian Ran Song; Yan Xia Wang 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 807 KB

## Abstract In this study a three step culture system, 2D‐3D sequential culture __in vitro__ and further implantation __in vivo__ was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five‐day‐old embryoid bodies were plated for chondrogenic induction for 27 days (