A novel, high stringency selection system allows screening of few clones for high protein expression
β Scribed by H.J.M. van Blokland; T.H.J. Kwaks; R.G.A.B. Sewalt; J.A. Verhees; V.N.A. Klaren; T.K. Siersma; J.W.M. Korse; N.C. Teunissen; S. Botschuijver; C. van Mer; S.Y. Man; A.P. Otte
- Book ID
- 111657437
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 528 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0168-1656
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## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo