Transgenic mutation assays generally use mutant frequencies to estimate mutation frequencies but the degree to which clonal expansion inflates mutant frequencies i s largely unknown. Mutant frequency is defined as the fraction of cells carrying mutations in the gene of interest and, according to the
A novel germline mutation in Big Blue® mice
✍ Scribed by Rory A. Crabbe; Anita Prtenjaca; Heather E. Tarnowski; Kathleen A. Hill
- Book ID
- 102186012
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 165 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0893-6692
- DOI
- 10.1002/em.20442
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Endogenous oxidative DNA damage caused by normal cellular processes may play a vital role in carcinogenesis. To directly test the hypothesis that antioxidants will protect DNA from oxidative damage in vivo, Big Blue ® mice were fed either a control diet (66 IU vitamin E/kg diet) or a highdose vitami
## Abstract Transgenic mouse mutation detection systems permit rapid determination of the frequency and type of mutations allowing direct examination of mutational markers for aging, neurodegeneration, and cancer. The Big Blue® transgenic mouse mutation detection system was used to determine the fr