A novel fluorometric assay for ADP-ribose pyrophosphatase activity
β Scribed by Eun-Kyung Song; Hyung-Ju Park; Jong-Suk Kim; Hwang-Ho Lee; Uh-Hyun Kim; Myung-Kwan Han
- Book ID
- 116616222
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 247 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0165-022X
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Poly(ADP-ribose) polymerase (PARP) is an abundant nuclear protein in most of the eukaryotic tissues. When activated by DNA damage, PARP synthesizes poly(ADP-ribose) from NAD. Conventional radioactive PARP enzyme assay requires the separation of the polymer product from the NAD substrate, a rate-limi
A fluorometric method for the assay of cholesterol reductase activity from pea leaves (Pisum sativum) is presented. This method is based on the decrease in relative fluorescence occurring as a result of the oxidation of NADH when cholesterol is reduced catalytically to coprostanol by cholesterol red