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A novel fluorescent assay used to measure the absorption efficacy of a sonic applicator


Book ID
116588331
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
49 KB
Volume
66
Category
Article
ISSN
1097-6787

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A sensitive competitive method was developed for assessing the reactivity of compounds toward glutathione and toward thiols in general. The method employs the reaction of the fluorogenic reagent fluorescein-5-maleimide (FM) with glutathione (GSH) to generate a large increase in fluorescence emission