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A novel fluorescence quantification method for polymerase chain reaction system

โœ Scribed by Jui Hung Chien; Da Sheng Lee; Yi Ting Cheng; Shou Huei Yeh; Wen Ping Chou; Ping Hei Chen

Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
462 KB
Volume
266
Category
Article
ISSN
0030-4018

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Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt