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A Novel Fluorescence Immunoassay System Based on pH-Sensitive Phase Separating Technique

โœ Scribed by Lin Peng; Zheng Hong; Yang Huang-Hao; Li Dong-Hui; Xu Jin-Gou


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
70 KB
Volume
23
Category
Article
ISSN
0256-7660

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Fluorescence Immunoassay System Based on
โœ Huang-Hao Yang; Qing-Zhi Zhu; Shi Chen; Dong-Hui Li; Xiao-Lan Chen; Ma-tai Ding; ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 83 KB

Poly(N-isopropylacrylamide-co-methacrylic acid) [P(NIPAAm-co-MAA)], a linear water-soluble pH-sensitive phase-separating polymer, was synthesized and used as a novel separation carrier for the reactants in immunoassay. This polymer precipitates out of water below a critical pH 5.8 at 37ยฐC and rediss