A novel expression vector for transfection of bovine MHC class I genes
โ Scribed by Suminder M.S. Sawhney; John Hopkins; Roger L. Spooner; George C. Russell
- Book ID
- 116133587
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 400 KB
- Volume
- 171
- Category
- Article
- ISSN
- 0378-1119
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Mouse Sal sarcoma cells are lethal in the autologous A/J (K") host. In order t o improve the immune response t o the Sal tumor, Sal cells have been transfected with syngeneic MHC-class-ll or allogeneic MHC-class4 genes. MHC-class-ll transfectants are uniformly rejected by the autologous host and imm
A cDNA library prepared from lymphocytes of a cow (E98), homozygous at major histocompatibility complex (MHC) loci (BoLA phenotype w10, KN104), was screened with a bovine MHC class I probe. Of the cDNA clones isolated, two, (2.1 and 5.1) were selected and showed divergence at both 5' and 3' termini.