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A novel expression system for intracellular production and purification of recombinant affinity-tagged proteins inAspergillus niger

✍ Scribed by Andreas H. F. J. Roth; Petra Dersch

Publisher
Springer
Year
2009
Tongue
English
Weight
489 KB
Volume
86
Category
Article
ISSN
1432-0614

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## Abstract A multiple vector system for the intracellular high‐level production of affinity tagged recombinant proteins in __Bacillus megaterium__ was developed. The N‐ and C‐terminal fusion of a protein of interest to a Strep II and a His~6~‐tag is possible. Corresponding genes are expressed unde