A novel exogenous mammary tumor virus encoding MHC class II H2E-independent superantigen specific foor Tcr-Vβ14
✍ Scribed by Worawidh Wajjwalku; Yoshihiro Ando; Naoya Niimi; Yasunobo Yoshikai
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 278 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0093-7711
No coin nor oath required. For personal study only.
✦ Synopsis
Similar to the superantigens encoded by endogenous mouse mammary tumor virus (MMTV) proviruses (Acha-Orbea et al. 1993), infectious MMTVs express superantigens, which are recognized by particular Tcrb-V in the context of MHC class II molecules (Marrack et al. 1991). Choi and coworkers (1991) and Acha-Orbea and co-workers (1991) have reported independently that MMTV(C3H) and MMTV(GR) encode superantigens which interact with T cells expressing Tcr-V~314. Both retroviral superantigens require exclusively MHC class II H2E expression for presentation and clonal deletion, and do not induce vigorous T-cell proliferation (Held et al. 1992).
We injected partially purified virus particles from the milk of RIII mice or II TES mice, which had been established by the crossbreeding of DBA/2 with strains of Japanese pet mouse origin (NBCS-II and CS), into the footpad of adult BALB/c or C57BL/6(B6), and the V~3 repertoire was examined in the popliteal LN cells 4 days after injection by flowcytometry. As shown in Figure 1, the V~314 T cells in CD4 + LN cells were increased in BALB/c mice 4 days after injection with the II-TES or RIII milk, while V[36 T cells were decreased in these mice, presum-