A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors

✍ Scribed by Y.I Korpan; V.V Volotovsky; C Martelet; N Jaffrezic-Renault; E.A Nazarenko; A.V El'skaya; A.P Soldatkin

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 71 KB
• Volume: 55
• Category: Article
• ISSN: 1567-5394
• DOI: 10.1016/s1567-5394(01)00150-5

✦ Synopsis

For the design of a biosensor sensitive to steroidal glycoalkaloids, pH-Sensitive Field Effect Transistors as transducers and immobilised butyrylcholinesterase as a biorecognition element have been used. The total potato glycoalcaloids can be measured by this biosensor in the concentration range 0.5 -100 mM with detection limits of 0.5 mM for a-chaconine and of 2.0 mM for a-solanine and solanidine, respectively. The responses of the developed biosensors were reproducible with a relative standard deviation of about 1.5% and 5% for intra-and intersensor responses (both cases, n = 10, for an alkaloid concentration of 5 mM), respectively. Moreover, due to the reversibility of the enzyme inhibition, the same sensor chip with immobilised butyrylcholinesterase can be used several times (for at least 100 measurements) after a simple washing by a buffer solution and can be stored at 4 °C for at least 3 months without any significant loss of the enzymatic activity.