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A Novel Enzymatic Method for Measuring Mizoribine 5′-Monophosphate Levels in Serum

✍ Scribed by Hiroko Ota; Yuko Yasuda; Shin-Ichi Sakasegawa; Shigeyuki Imamura; Tomohiro Tamura

Book ID
118729470
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
263 KB
Volume
106
Category
Article
ISSN
1389-1723

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✍ Douglas A. Young; Joyce A. Carter; Maggie M.-Y. Chi; Oliver H. Lowry 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 376 KB

A method for measuring inosine 5'-monophosphate (IMP) by enzymatic generation of NADPH is described. Procedures are given for direct fluorometric assay in the nanomole range and indirect measurement with amplification by enzymatic cycling in the pico- and femtomole ranges. The most sensitive procedu