A novel electrochemiluminescence glucose biosensor based on alcohol-free mesoporous molecular sieve silica modified electrode
✍ Scribed by Rong Lei; Xinyi Wang; Shuifang Zhu; Na Li
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 561 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0925-4005
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✦ Synopsis
In this study, a thin-film glucose electrochemiluminescence (ECL) biosensor was developed. Ru(bpy) 3 2+ was doped in alcohol-free low-volume shrinkage mesoporous silica sol-gel with PEG-400 as the template, and glucose dehydrogenase (GDH) was immobilized in polymer Resydrol. NADH, which is produced by the reaction of co-enzyme NAD + and glucose catalyzed by glucose dehydrogenase, reacts with immobilized Ru(bpy) 3 2+ to generate ECL emission. Among the three types of design including two-layer, mixed and sandwich configuration, the sandwich configuration showed the best sensitivity with the calibration range of 25-2000 M and the limit of detection of 0.5 M in a flow injection analysis of glucose. The alcohol-free mesoporous sol-gel and the sandwich design made the biosensor potentially applicable in flow-injection analysis of samples.