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A novel dot-blot DNAzyme-linked aptamer assay for protein detection

✍ Scribed by Jinbo Zhu; Tao Li; Jiming Hu; Erkang Wang

Book ID
105893324
Publisher
Springer
Year
2010
Tongue
English
Weight
220 KB
Volume
397
Category
Article
ISSN
1618-2650

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A method for the detection and quantitation of picomole amounts of heparin-binding proteins is described. Proteins are first spotted on nitrocellulose and then incubated with "51-heparin. Binding of heparin to the proteins is detected by radioautography and quantitated by scanning densitometry; pro