A novel detection method for DNA point mutation using QCM based on Fe3O4/Au core/shell nanoparticle and DNA ligase reaction

A Sensitive Fluorescence Anisotropy Meth

A Sensitive Fluorescence Anisotropy Method for Point Mutation Detection by Using Core–Shell Fluorescent Nanoparticles and High-Fidelity DNA Ligase

✍ Ting Deng; Jishan Li; Jian-Hui Jiang; Guo-Li Shen; Ru-Qin Yu 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 278 KB 👁 1 views

## Abstract The present study reports a proof‐of‐principle for a sensitive genotyping assay approach that can detect single nucleotide polymorphisms (SNPs) based on fluorescence anisotropy measurements through a core–shell fluorescent nanoparticles assembly and ligase reaction. By incorporating the