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A novel competitive fluorescence immunoassay for the determination of dibutyl phthalate

โœ Scribed by Ming-Cui Zhang; Qiong-E Wang; Hui-Sheng Zhuang

Publisher
Springer
Year
2006
Tongue
English
Weight
125 KB
Volume
386
Category
Article
ISSN
1618-2650

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We describe the use of a thermostable glucokinase in a novel competitive fluorescence assay for glucose. Glucokinase from Bacillus stearothermophilus (BSGK) was found to retain enzymatic activity in solution for over 20 days. The single cysteine residue in BSGK, which is near the active site, was la