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A novel chromosomal region of allelic loss, 4q32–q34, in human osteosarcomas revealed by representational difference analysis

✍ Scribed by Annet Simons; Marga Schepens; Anne Forus; Linda Godager; Martien van Asseldonk; Ola Myklebost; Ad Geurts van Kessel

Book ID: 101263523
Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 131 KB
Volume: 26
Category: Article
ISSN: 1045-2257
DOI: 10.1002/(sici)1098-2264(199910)26:2<115::aid-gcc3>3.0.co;2-e

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✦ Synopsis

Representational difference analysis (RDA) of a human osteosarcoma xenograft resulted in the isolation of four tumorassociated homozygously deleted DNA fragments, all originating from chromosome 4, region q32-q34. Southern blot analysis using the RDA fragments and interphase FISH analysis using PACs corresponding to these RDA fragments revealed allelic loss of the 4q32-q34 region in 17 of 27 (63%) osteosarcomas tested. These results suggest the involvement of tumor suppressor gene(s) within this chromosomal region in osteosarcoma development. The RDA fragments and corresponding PAC clones will be instrumental in the isolation of such gene(s).

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A novel region of amplification at 11p12

A novel region of amplification at 11p12-13 in gastric cancer, revealed by representational difference analysis, is associated with overexpression of CD44v6, especially in early-onset gastric carcinomas

✍ Ralph Carvalho; Anya N. A. Milne; Mirjam Polak; G. Johan A. Offerhaus; Marian A. 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 348 KB 👁 1 views

## Abstract Diffuse‐type gastric carcinomas (GCs) are often difficult to characterize because of contamination of tumor samples by surrounding normal tissue. As such, information regarding chromosomal aberrations in this subtype of GCs is limited. In this study, we used representational difference