A Novel Chemiluminescence Metalloimmunoassay Based on Gold Label

✍ Scribed by Aiping Fan; Choiwan Lau; Zhixin Liao; Jianying Liang; Jianzhong Lu

• Book ID: 104452393
• Publisher: Elsevier Science
• Year: 2006
• Tongue: Chinese
• Weight: 149 KB
• Volume: 34
• Category: Article
• ISSN: 1872-2040
• DOI: 10.1016/s1872-2040(06)60005-4

✦ Synopsis

A novel chemiluminescence (CL) metalloimmunoassay based on the dissolution of gold nanoparticles has been presented as demonstrated by the determination of biotinylated antibody and IgG. The experimental conditions such as gold dissolution and CL reaction were optimized. In the detection format of primary antibody-IgG-antibody modified gold (10 nm and 30 nm gold), the dynamic range of IgG extended between 1-75 ng and 0.5-25 ng, respectively. The detection limit was estimated to be 0.5 ng for 10 nm of gold and 0.1 ng for 30 nm of gold. In the detection format of primary antibody-IgG-biotinylated antibody-streptavidin gold (5 nm and 10 nm), the dynamic range of IgG extended between 10-250 ng and 1-250 ng, respectively. The detection limit was estimated to be 5 ng for 5 nm of gold and 1 ng for 10 nm of gold. Overall, this novel method can be readily extended to a large variety of clinical bioaffinity assays of analysis and the detection of DNA hybridization.