A novel cereal storage protein: molecular genetics of the 19 kDa globulin of rice
✍ Scribed by Basil S. Shorrosh; Lisa Wen; Kuo-Chang Zen; Jenq-Kuen Huang; Jean S. Pan; Mark A. Hermodson; Kunisuke Tanaka; Subbaratnam Muthukrishnan; Gerald R. Reeck
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 315 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
A 2gtl 1 cDNA library, constructed from poly(A) + RNA isolated from immature rice seed endosperm, was screened with affinity-purified antibodies against the rice storage protein called e-globulin (previously), or the 19 kDa globulin (our term). A positive clone was isolated and sequenced and shown to encode a 21 kDa precursor for the 19 kDa globulin, based on the identity of portions of the inferred amino acid sequence and the sequence of three cyanogen bromide peptides of the 19 kDa globulin. Analysis of genomic DNA by Southern blotting using the cDNA clone probe revealed one hybridizing band in