A novel biosensor for bovine serum albumin based on fluorescent self-assembled sandwich bilayers

✍ Scribed by Xiangying Sun; Bin Liu; Fei He

• Book ID: 101714204
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 263 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1066

✦ Synopsis

Abstract

Fluorescent dyes butyl rhodamine B were assembled via a dl‐cystenine intermediate onto quartz wafers whose surface had first adsorbed gold nanoparticles. Hence self‐assembled sandwich bilayers with nanocomposite structure were constructed which can be used as a biosensor for bovine serum albumin. The biosensor‐based self‐assembled monolayers (SAMs) are regenerable and have high sensitivity, five orders of magnitude higher than that of bulk solution phase sensing. The effects of existing forms of dyes on the fluorescence spectra of bilayers in the presence of bovine serum albumin were investigated. Copyright © 2008 John Wiley & Sons, Ltd.