## Abstract ## Background Various protocols for estimation of telomere length in individual cells by flow cytometry using fluorescence in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes (FlowβFISH) have been described. Combined analysis of telomere length and cell phe
A novel B cell stimulation/proliferation assay using simultaneous flow cytometric detection of cell surface markers and DNA content
β Scribed by Jan Storek; Ingrid Schmid; Stephen Ferrara; Andrew Saxon
- Book ID
- 118902561
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 284 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0022-1759
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## Abstract This paper describes the measurement of S phase DNA content in human bone marrow subpopulations using a single laser method for bivariate analysis of DNA content and cellβsurface immunofluorescence (sβIF). Low density (<1.077 g/ml) bone marrow cells were labeled with a panel of unconjug
We have designed an assay for the simultaneous measurement of cell surface phenotype, S-phase fraction, and DNA content by single laser instrumentation for the purpose of determining the labeling index (LI), duration of S-phase (Ts), and the potential doubling time (Tpot) of leukocyte subpopulations