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A novel approach to the generation of seamless constructs for plant transformation

โœ Scribed by Kronbak, Remy; Ingvardsen, Christina; Madsen, Claus; Gregersen, Per

Book ID
125402510
Publisher
BioMed Central
Year
2014
Tongue
English
Weight
722 KB
Volume
10
Category
Article
ISSN
1746-4811

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The loxP-Cre site-specific recombination system of phage P1 was used to develop a novel strategy to construct cointegrate vectors for Agrobacterium-mediated plant transformation. A pTi disarmed helper plasmid (pAL1166) was constructed by replacing the oncogenic T-DNA by a loxP sequence and a spectin