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A novel approach to the detection of estrogen receptors in bone cells

✍ Scribed by T. Stulc; P. Kruzik; D. Klement; J. Kvasnička; J. Štěpán

Book ID: 105599353
Publisher: Springer-Verlag
Year: 1996
Tongue: English
Weight: 129 KB
Volume: 6
Category: Article
ISSN: 0937-941X
DOI: 10.1007/bf02500424

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Expression of isoforms of estrogen receptor (ER) was examined in the bone tissues. Reverse transcriptase-polymerase chain reaction ((RT-PCR) using specific primers for rat ER cDNA was performed with total RNA from rat bone tissues. Then, we sequenced the amplified products after cloning and identifi