A thermo-responsive polymer on which maltose was covalently immobilized as an affinity ligand was newly synthesized for purification of thermolabile proteins from the crude solution by affinity precipitation. Among the thermo-responsive polymers synthesized as carriers for adsorbent, poly(N-acryloyl
β¦ LIBER β¦
A novel application of thermo-responsive polymer to affinity precipitation of polysaccharide
β Scribed by Li-Chun Pan; Chih-Ching Chien
- Book ID
- 117331299
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 135 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0165-022X
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## Abstract A gradient micropattern immobilization technique using a photomask was developed to investigate by microscopic observation the effect of the surface concentration of an immobilized thermoβresponsive polymer. Poly(__N__βisopropylacrylamideβ__co__βacrylic acid) was chosen as the thermoβre