𝔖 Bobbio Scriptorium
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A novel affinity column for purification of glycerol phosphate dehydrogenase

✍ Scribed by James F. McGinnis; Jean de Vellis

Book ID
118309138
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
598 KB
Volume
60
Category
Article
ISSN
0006-291X

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An NAD-dependent glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ oxidoreductase, EC 1.1.1.8) has been isolated and purified from Saccharomyces cerevisiae by affinity and exclusion chromatography. The enzyme was purified 5 1 OO-fold to a specific activity of 158. It has a molecular w