✦ LIBER ✦

A novel adenovirus-based system for facilitating retroviral hepatic gene transfer in vivo

✍ Scribed by M Ott; S G Gupta

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 158 KB
Volume: 23
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.510230333

No coin nor oath required. For personal study only.

✦ Synopsis

A NOVEL ADENOVIRUS-BASED SYSTEM FOR limits the number of cells that may be transduced. Retroviral gene transfer also requires proviral integration

FACILITATING RETROVIRAL HEPATIC GENE TRANSFER IN VIVO

into the genome, which is greatly facilitated by DNA synthesis; however, hepatocytes are proliferatively qui-Lieber A, Vrancken Peeters M-JTFD, Meuse L, Fausto escent (only 0.005% of unperturbed hepatocytes may N, Perkins J, Kay MA. Adenovirus-mediated urokinase be in S-phase). For in vivo hepatic gene therapy, DNA gene transfer induces liver regeneration and allows for synthesis is induced at present by partial hepatectomy, efficient retrovirus transduction of hepatocytes in vivo. which is cumbersome and potentially risky. Also, liver

📜 SIMILAR VOLUMES

Hepatic delivery of particulates in the

Hepatic delivery of particulates in the submicron range by a hydrodynamics-based procedure: implications for particulate gene delivery systems

✍ Naoki Kobayashi; Kazuhiro Hirata; Shi Chen; Atsushi Kawase; Makiya Nishikawa; Yo 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 350 KB

## Abstract ## Background A large‐volume intravenous (i.v.) injection of DNA, i.e. a hydrodynamics‐based transfection procedure, is known to be an efficient and liver‐specific method of __in vivo__ gene delivery. However, little is available on an applicable particle size in the procedure. ## Met