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A novel acylated flavonol glycoside isolated from Brunfelsia grandiflora ssp. grandiflora. Structure elucidation by gradient accelerated NMR spectroscopy at 14T

✍ Scribed by Gerhard Brunner; Ulrich Burger; Pierre Castioni; Ilias Kapetanidis; Philippe Christen


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
70 KB
Volume
11
Category
Article
ISSN
0958-0344

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✦ Synopsis


Kaempferol

-3-O-[6 Glc -O-feruloyl]-b-D-glucopyranosyl-(132)-O-[a-L-rhamnopyranosyl-(136)]-b-D-galactopyranoside (1) was isolated from the aerial parts of Brunfelsia grandiflora ssp. grandiflora. The structure of this novel flavonol glycoside was established by fast atom bombardment-mass spectrometry and by means of NMR spectroscopy at 14T, using two-dimensional NMR experiments with pulsed-field gradients for coherence selection. The presence of the constituent aglycones and sugar units of 1 was confirmed independently by acid hydrolysis.