A novel acidotropic pH indicator and its potential application in labeling acidic organelles of live cells

✍ Scribed by Zhenjun Diwu; Chii-Shiarng Chen; Cailan Zhang; Dieter H Klaubert; Richard P Haugland

• Book ID: 104375815
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 804 KB
• Volume: 6
• Category: Article
• ISSN: 1074-5521
• DOI: 10.1016/s1074-5521(99)80059-3

✦ Synopsis

Conclusions: PDMPO selectively labels acidic organelles (such as lysosomes) of live cells and the two distinct emission peaks can be used to monitor the pH fluctuations of live cells in ratio measurements. Additionally, the very large Stokes shift and excellent photostability of PDMPO make the compound an ideal fluorescent acidotropic probe. The unique fluorescence properties of PDMPO might give researchers a new tool with which to study acidic organelles of live cells.