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A note on the enzymic action and biosynthesis of a nitrile-hydratase from aBrevibacteriumsp.

✍ Scribed by Bui, K. ;Maestracci, M. ;Thiery, A. ;Arnaud, A. ;Galzy, P.


Book ID
114735174
Publisher
Wiley (Blackwell Publishing)
Year
1984
Tongue
English
Weight
370 KB
Volume
57
Category
Article
ISSN
0021-8847

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Besides its nitrile hydratase and wide spectrum amidase activities, the Brevibacterium sp. R312 strain also possesses a constitutive beta-glucosidase. Its optimum pH is 6. The enzyme was purified by fractionation precipitation with ammonium sulfate followed by chromatographic elutions on Q-Sepharose