A Noncommercial Dual Luciferase Enzyme Assay System for Reporter Gene Analysis

✍ Scribed by Benjamin W. Dyer; Fernando A. Ferrer; Donna K. Klinedinst; Ronald Rodriguez

• Book ID: 102562960
• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 107 KB
• Volume: 282
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4605

✦ Synopsis

of the desired polymerase should be taken into consideration. 5N/1S primers are recommended for Taq, whereas 3N/1S primers should be used with Vent and Pfu polymerases.

Acknowledgments. We thank Dr. Ga ´bor Ra ´khely for his valuable help and Andrea Vo ¨ro ¨s for technical assistance.