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A Non-Enzymatic Method for the Isolation of Protoplasts From Callus of Saintpaulia ionantha (African Violet)

✍ Scribed by Bilkey, P.C.; Cocking, E.C.


Book ID
123219079
Publisher
Elsevier
Year
1982
Weight
424 KB
Volume
105
Category
Article
ISSN
0044-328X

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A method for isolation of rat hepatocytes using liver perfusion by ethylenediamine tetra-acetic acid (EDTA)-containing sucrose solution and mechanical tissue disaggregation by controlled vibration (MVD) is described. The yields of hepatocytes produced by this method were similar to those obtained us