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A new99mTc-red blood cell labeling procedure for cardiac blood pool imaging: clinical results

✍ Scribed by Henning Kelbæk; Kirsten Bülow; Jan Aldershvile; Jens Møgelvang; Steen Levin Nielsen

Publisher
Springer
Year
1989
Tongue
English
Weight
279 KB
Volume
15
Category
Article
ISSN
0340-6997

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✦ Synopsis

The first clinical results of a new 99mTc-red blood cell labeling procedure avoiding cell centrifugation are presented. One ml heparinized blood samples were incubated with small amounts of a stannous kit. By titration studies, ideal quantities of sodium hypochlorite for oxidation of extracellular tin and of EDTA as stabilizer of the label were found. The C1-concentration and pH of the labeled blood were acceptable, and EDTA increased labeling yield and stability determined in vitro by a few percent. The new procedure gave a slightly higher labeling yield than a current technique using centrifugation of cells. Labeling efficiency expressed as cell bound/total activity was 96.6% _+ 1.3% in healthy subjects and 95.5% +2.2% in cardiac patients and remained high for 2 h after reinjection. The biological halflife of labeled cells following the new procedure was 11-12 h rendering it suitable for serial determinations of radionuclide cardiography.

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