✦ LIBER ✦

A new type of capillary column for open-tubular electrochromatography

✍ Scribed by Yingxin Zhao; Rui Zhao; Dihua Shangguan; Guoquan Liu

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 119 KB
Volume: 23
Category: Article
ISSN: 0173-0835
DOI: 10.1002/1522-2683(200209)23:17<2990::aid-elps2990>3.0.co;2-7

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✦ Synopsis

A new type of open-tubular C(18) ester-bonded electrochromatographic column was prepared with sol-gel technology, followed by an on-column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol-gel precursor to form a thin coating layer on the wall of the fused-silica capillary. The C(18) groups were introduced into the coating layer by on-column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8x10(5) plates/m was achieved for a basic pentapeptide using the C(18 )ester-bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol-gel-coated capillaries, the C(18) ester-bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage.

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