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A new three allele polymorphism at distal 21q22.3, a region relatively devoid of polymorphic markers

✍ Scribed by Andréa L. Sertié; Christina Brahe; M. Rita Passos-Bueno

Book ID
101260367
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
16 KB
Volume
13
Category
Article
ISSN
1059-7794

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✦ Synopsis

We amplified 305bp of the 3'UT region of COL18A1 from 62 healthy chromosomes from Caucasian individuals. SSCP analysis of these PCR products allowed us to identify 3 alleles which were confirmed through sequencing analysis and ASO. The heterozigosity found for this polymorphic region is 74,2%. and mendelian inheritance was demonstrated in 5 two/three generation families. This polymorphism, located in a region relatively devoid of microsatellites, will be very useful for linkage analysis, particularly for narrowing down candidate regions for genes mapped in this area.

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