A new technique for highly sensitive detection of superoxide dismutase activity by chemiluminescence

✍ Scribed by Philippe Corbisier; Andree Houbion; Jose Remacle

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 505 KB
• Volume: 164
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90392-7

✦ Synopsis

A stable enzymatic free radical generation system has been developed which allows a precise production of 05 and its detection by chemiluminescence between 2 pmol and 8 nmol. This test has been used for assaying superoxide dismutase (SOD) by inhibition of the chemiluminescence (CL) signal. No inhibition was observed with catalase, which excludes the participation of Hz02 in lucigenin CL. N,N-Diethyldithiocarbamate gives 100% inhibition of SOD activity either from a purified enzymatic preparation or from biological samples, which confirms the specificity of the CL assay. SOD assay can be performed either on a purified enzymatic preparation or on biological materials such as cultured cells. 0 1987 Academic press, IIIC.