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A new system for highly efficient generation of alloantigen-specific cytotoxic T cells

✍ Scribed by Linda M. Pilarski; Ludmilla V. Borshevsky

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
464 KB
Volume
6
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

A tissue culture system for generating alloantigen‐specific cytotoxic T cells is described which utilizes Marbrook polyacrylamide rafts as the culture vessel. The system has the following properties.

Cytotoxic T cells are generated after culture of very low numbers of responder lymphocytes. Cultures routinely contain 0.5 × 10^6^ ‐ 1 × 10^6^ responder cells but as few as 3 × 10^4^ lymphocytes/culture generate significant cytotoxicity.

Generation of cytotoxicity is efficient at all cell doses, producing killer cells effective at lymphocyte‐to‐target ratios of from 0.03: 1 to 5:1. The lowest ratio represents cytotoxicity by the progeny of only 833 lymphocytes.

)The number of cells in cultures increases rather than decreases over the five‐day culture period yielding a 2–4‐fold average increase in viable cells.

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