A new strategy to screen MMR genes in Lynch Syndrome: HA-CAE, MLPA and RT-PCR

Quantitative PCR high-resolution melting

Quantitative PCR high-resolution melting (qPCR-HRM) curve analysis, a new approach to simultaneously screen point mutations and large rearrangements: application to MLH1 germline mutations in Lynch syndrome

✍ Etienne Rouleau; Cédrick Lefol; Violaine Bourdon; Florence Coulet; Tetsuro Noguc 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 427 KB 👁 1 views

Several techniques have been developed to screen mismatch repair (MMR) genes for deleterious mutations. Until now, two different techniques were required to screen for both point mutations and large rearrangements. For the first time, we propose a new approach, called "quantitative PCR (qPCR) high-r