𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new strategy for the on-column exopeptidase cleavage of poly-histidine tagged proteins

✍ Scribed by Wen-Hui K. Kuo; Howard A. Chase

Book ID
116620546
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
566 KB
Volume
879
Category
Article
ISSN
1873-376X

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Process intensification for the removal
Process intensification for the removal of poly-histidine fusion tags from recombinant proteins by an exopeptidase
✍ Wen-Hui K. Kuo; Howard A. Chase πŸ“‚ Article πŸ“… 2009 πŸ› American Institute of Chemical Engineers 🌐 English βš– 240 KB πŸ‘ 1 views

## Abstract This study describes the use of a hexa‐histidine tagged exopeptidase for the cleavage of hexa‐histidine tags from recombinant maltose binding protein (MBP) when both tagged species are bound to an immobilized metal affinity chromatography (IMAC) matrix. On‐column exopeptidase cleavage o