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A New Strategy for Site-Specific Protein Modification: Analysis of a Tat Peptide−TAR RNA Interaction

✍ Scribed by Tamilarasu, Natarajan; Zhang, Jing; Hwang, Seongwoo; Rana, Tariq M.


Book ID
120536392
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
118 KB
Volume
12
Category
Article
ISSN
1043-1802

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Interference of binding of Tat protein to TAR RNA in HIV-1-infected cells may be a useful therapeutic strategy for AIDS. An electrophoretic assay to screen potential low-molecular-weight (< 2 kDa) Tat antagonists has been established. A radiolabeled TAR RNA fragment (delta TAR) is retarded in mobili