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A new sensitive radial diffusion method for microdetermination of α-amylase

✍ Scribed by Horst-Dietmar Tauschel; Claus Rudolph

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
553 KB
Volume
120
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive agarose diffusion method for the determination of cY-amylase has been developed, using Reactone Red 2 B-amylopectin as the substrate. The logarithm of enzyme activity is linearly correlated with the diameters of the diffusion zones over a very extended range from 1 mu/ml to at least 1100 U/ml. The Lu-amylase activity in biological samples may be determined without dilution or pretreatment, and the test can be performed at any desired temperature between 4 and 45°C. The clear radial diffusion zones may be fixed, further enhancing the contrast to the bright red surrounding.

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