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A new reaction scheme for the alkaline isomerization of horseradish peroxidase

✍ Scribed by Hiroshi Kihara; Satoshi Saigo; Tetsutaro Iizuka; Yuzuru Ishimura

Book ID
113125888
Publisher
Elsevier Science
Year
1978
Weight
435 KB
Volume
533
Category
Article
ISSN
0005-2795

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N,NЈ-Dicyanomethyl-o-phenylenediamine was synthesized with a 90% yield by a reaction of o-phenylenediamine with chloroacetonitrile in triethylamine. Our experimental results showed that it was the effective fluorogenic substrate for horseradish peroxidase (HRP) and hemin. The K m for the HRP system