A new quantitative assay for glycosaminoglycans
โ Scribed by Le Phuc Thuy; W.L. Nyhan
- Book ID
- 115827451
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 701 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0009-8981
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple and reliable method is described for the determination of glycosaminoglycans and proteoglycans in tissue extracts as well as during preparative and analytical procedures for these molecules. It is particularly useful because it requires much less starting material for the identification of
A method is described for the assay of sulfotransferases, which transfer sulfate from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to glycosaminoglycan acceptors. Following the sulfation reactions, the [35S]sulfate-labeled products are precipitated and then separated from a sulfate donor ([35S]PAPS)