✦ LIBER ✦
A new principle for activity measurement of ADP or ATP dependent enzymes: Fluorescence quenching of ϵ-ADP and ϵ-ATP by divalent metal ions
✍ Scribed by Wolfgang Ernst Höhne; Peter Heitmann
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 581 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The divalent metal ions Cu'+, Co 2+, Mn2+, and Zn*+ form complexes with the fluorescent etheno analogs of the adenine nucleotides. The fluorescence intensity is thereby diminished. The binding strength of the metals to etheno-adenosine triphosphate is higher than to etheno-adenosine di-and monophosphate. The quenching effect of the divalent metal ions can be exploited as a simple routine activity measurement for various kinases and phosphohydrolases.