A new polymorphism in the human factor VIII gene: implications for linkage analysis in haemophilia A and for the evolution of int22h sequences
β Scribed by Derrick J. Bowen; Carlos D. De Brasi; Irene B. Larripa; Peter W. Collins
- Book ID
- 108672376
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 141 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0007-1048
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A combination of Southern blotting and the analysis of polymerase chain reaction (PCR) amplified DNA fragments was used to screen the factor VIII genes of 527 haemophilia A patients for point mutations within TaqI restriction sites. Since this "directed search" strategy yielded only four gene lesion