A new mutator strain of Bacillus subtilis

✍ Scribed by Viret, Jean-François ;Alonso, Juan Carlos

• Book ID: 104725125
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 418 KB
• Volume: 208
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00330465

✦ Synopsis

Bacillus subtilis strain SB1207, widely used in our laboratory, was found to be highly temperature-sensitive and to exhibit a strong SOS-independent mutator phenotype at elevated temperatures. Both chromosomal and plasmid-borne genes were affected by the mutator. Lethality and mutator phenotype could not be attributed to a replication shut off or to thymine starvation. Due to the high frequency of base misincorporation, the mutator phenotype probably results from an editing defect rather than from a post-replication defect (mismatch repair).